Lipase Producing Rhodococcus Strains Analysis

Lipase Producing Rhodococcus Strains Analysis CHAPTER 1 INTRODUCTION Overview of research Malaysia has attained an outstanding position as world’s second largest palm oil producer. This remarkable quality indirectly eases high consumption of palm oil in the country. As per statistic, industrial domestic consumption has achieved 2,000 million tonnes of palm oil while, general domestic consumption reaches 3,160 million tonnes for the year 2013 (Index Mundi, 2013). Considering variable food industry scales available in the country, this ideal identity has even manifested Malaysia’s status as â€Å"Asia’s Food Paradise†. Number advantages such wide availability and consistent utilization of cooking oil throughout the nation, has indirectly resulted to startling questions on the management of used cooking oil. Coined and defined already that, lipids holds the largest portion of organic components in municipal and industrial wastewater particularly from food industries (Prasad Manjanuth, 2011). Improper management of fatty wastes on the other hand, has led to serious anthropogenic damages such as clogging pipes, congest treatment filters, odour problems, oil film formation and depleting oxygen hence, damaging aquatic livings (Fadile et al., 2011; Cipinyte et al., 2009). Therefore, removal as well as early remediation on lipid contaminant has become a great necessary due to its associated negative impacts. In regards to the necessity of improvement in biological treatment, enzymatic technology specifically lipase is gaining increasing attention. Concept of â€Å"White Biotechnology† introduces two implications on fatty acid contaminants namely concept of biodegradation and biotransformation (Dors et al., 2013; Whiteley Lee, 2006). Biological treatment on lipid contaminated sites employ microbial lipase to detoxify, solubilize fatty acids and further commence conversion into microbial biomass. Despite the nature of treatment technology, it is crucial to ensure incorporation of right microbes on right place with suitable environment conditions for degradation to occur (Ugukchokwu et al., 2008). Fascinatingly, aerobic biodegradation by active lipolytic strains was proven to be feasible with many other added advantages compared to other strategies (Cipinyte et al., 2009). On top of it, lipases also exhibit excellent feasibility in early remediation through biotransformation approach. Abundant waste fatty feedstock prior to disposal can be modified in structure by lipolytic reactions. These modifications could be applied to serve demands for numerous industrial products like pet food, cosmetics, detergents, soap, floatation reagent and handcrafts (Used cooking oil, 2011; Shimizu, 1988; Alonso et al., 2000; Emoterabear, 2013). To date, current growing industrialization projected great necessary for alternative energy fuel-source due to depleting petroleum based fuels. As an enzymatic early remediation, researchers also have introduced lipase catalysed biodiesel product in oleochemical industry (Felizardo et al., 2006). Parallel to the â€Å"Green Environment† strategy, used cooking oil are proven to be possible alternate feedstock for biodiesel production as well (Chetri Watts et al., 2008). Considering wide range of versatility of lipase as biocatalyst, this enzyme is catered for both biodegradation and biotransformation productively. However, researchers are still in search of new lipase-lipid interaction to diversify the efficiency and upgrade quality of the existing outcome. Therefore in present investigation, production of lipase enzyme from locally isolated actinomycete known as Rhodococcus sp. was investigated. Denoting minimal documentation on lipase production by this actinomycete, yet the extreme ability of the strain to degrade oil extensively draws great intuition on lipase production. 1.2 Statement of problem As an amalgamation of many races, Malaysia has established a robust position for various food cuisines especially variable deep fried dishes. Therefore, large amount of cooking oil is required in the food preparation which simultaneously yields similar portion of utilized cooking oil. Consequently, excessive increments of waste cooking oil (WCO) are being disposed to waste streams continuously which lead to many detrimental effects to the environment. Therefore, it’s absolutely necessary to apply bioremediation concept to comprehensively treat the contaminated waste sites while providing early remediation to manage the increasing abundance of fatty waste materials. In order to emphasize high occurrence of waste cooking oil production especially in Malaysia, a survey was conducted in Teluk Bahang, Pulau Pinang, a local area popularly known for food business. The outcome reveals that the community generates 40% of more than 15kg of waste cooking oil monthly as described in Figur e 1.1. Figure 1.1: Production of WCO in Teluk Bahang, Pulau Pinang, a popular food industry area in Malaysia (Hanishah et al., 2013). 1.3 Justification of problem As aforementioned, cooking oil has become a basic ingredient for food preparation in the entire world. Deep frying is recognized as traditional practice of food preparation, where the oil are left exposed repeatedly to excessive heat of 160  ºC- 190  ºC in presence of moisture and air (Kamisah et al., 2012). Additionally, people tend to be fonder of fried food due to the attractive colour, fast preparation, delicious taste and catchy presentation in dining (Cvengros Cvengrosova, 2004). As a result, waste cooking oil are extensively being generated due to food and semi product preparation by frying such as fast food networks, restaurants, dining rooms, catering establishments throughout the world (Cvengros Cvengrosova, 2004; Kulkarni Dalai, 2006). According to a report by Capital Region District, 2012, almost 1million kilograms of fat contaminants are being disposed through our home drainage every year. Review in Asia countries like Malaysia, China, Indonesia, India and Thailand on the other hand, estimates to produce waste cooking oil for 40 000 tonnes per year (Hanisah et al., 2013). Meanwhile to be detail, excessive heating of cooking oil causes chemical modification by forming toxic compounds like peroxides, aldehyde and polymer via few reactions including hydrolysis, oxidation and thermolytic (Kulkarni Dalai, 2006; Hanisha et al. 2013). In agreement to this, study by Kamisah et al. (2012) found out that repeated heating of cooking oil augmented lipid peroxidation formation in the oil which causes severe health problems to human. Malaysian’s night markets for an instance, offer variety deep fried snacks such as keropok lekor, meat balls, sausages and potato chips. Different type of fried food indeed causes different fatty acid composition and anti- oxidant properties to the frying oil (Awney, 2011, Purushothama et al., 2003; Adam et al., 2007). In case of keropok lekor, a popular snack in Malaysia, the oil is prone to autoxidation due to the presence of polyunsaturated fatty acid (PUFA), therefore, the oil has been chemically modified (Kamsiah et al., 2 012). Nevertheless, if this â€Å"dead oil† been disposed to water streams or land, it indirectly causes both water pollution and land pollution. After being disposed to aquatic environment, this contaminant undergoes four natural actions namely weathering, evaporation, emulsification or biodegradation. In exceptional to biodegradation, the rest of natural actions leads to the formation of oil slick or oil film on surface of water. Meanwhile droplets of oil will be vertically distributed in water, increasing BOD and COD value besides, transforming into toxic compound due to degraded oil by-product (Hanisah et al., 2013; Understanding oil spills and oil spill response, 1999; Marjadi Dharaiya, 2010). Riskily, these cause an interaction between the oil and sediments to occur hence, affecting living of aquatic lives. Indirectly, these carcinogenic compounds can be absorbed into by marine creatures and affect human food chain (Kulkarni Dalai, 2006; Hanisah et al., 2013; Understanding o il spills and oil spill response, 1999). Figure 1.2 precisely describes natural actions encountered due to disposal of waste cooking oil into water and land. 1.4 Scope of research In regards to detrimental impacts imposed by waste cooking oil, it’s absolutely prudent to commence treatment on lipid contaminated sites and further discover feasible ways to manage the fatty waste materials. Therefore, presence research recommends remediation through biodegradation and biotransformation for an effectual management of the waste cooking oil. Lipases, being a ubiquitous biocatalyst significantly extend frontiers of applications through hydrolysis as well as esterification reactions. Favourably, waste cooking oil that has been disposed to the water streams can be potentially treated with biocatalytic action of lipase producing microorganisms. Waste cooking oil prior to disposal also can be potentially transformed into many value-added products through structure modification by the same catalyst as well. Concerning numerous microbial lipases and wide range of reactions catered by lipase enzyme, it’s essential to obtain this biocatalyst from a novel and reliable source. This will highlight new lipid-lipase interaction with better characteristics for improvement and further add intrinsic value for industrial interests adequately (Jesus et al., 1999; Glogauer et al., 2011). Considering the alarming production of waste cooking oil by local food industries and household disposal, a feasible remediation requires a promising local yet new source for lipase enzyme. Appropriately, local Rhodococcus strains, an actinomycete isolated throughout Peninsular Malaysia was not been evaluated for lipase production. Being an excellent hydrocarbon degrader, these serial of strains is expected to project excellent level of lipase secretion and further be applied into both biodegradation and biotransformation. Upon, identification of lipase production from this actinomycete, the best lipase producing strains was optimized for maximum enzyme activity. Since, this bacteria is poorly studied, the crude enzyme was subjected for electrophoresis to estimate the size protein band of the extracted enzyme. Later on, practicability of the optimized strains was applied as both whole cell biocatalyst (naturally immobilised cell) and immobilised intracellular crude. A preliminary analysis of the enzyme to biodegrade WCO and also biotransform WCO into biodiesel was evaluated also for future studies. Precisely, lipolytic actions illustrated in Figure 1.3 are the reactions evaluated in biodegradation and biotransformation of WCO by Rhodococcal lipase in this study. Figure 1.3: (a) Hydrolytic reactions displayed by lipase enzyme to solubilize fatty acids and further convert into biomass during biodegradation of WCO (Jaeger Reetz, 1998). (b) Trans-esterification reaction by lipase enzyme involving transfer of alcohol moiety to form acid alkyl ester during biotransformation in biodiesel production (Murugesan et al., 2009). 1.5 Objectives of this research The primary goal of this study is to identify and evaluate lipase producing Rhodococcus strains, as ideal source for bioremediation purposes. Following are the stages of objectives obeyed in this investigation: To screen and identify for the most potential lipase producing Rhodococcus isolates To study the optimum conditions required for maximum lipase production by identified lipolytic Rhodococcus sp. To evaluate ability of Rhodococcus lipase to degrade waste cooking oil. To investigate the ability of Rhodococcus lipase to catalyze conversion of waste cooking oil into biodiesel. Figure 1.4: Flow chart representing designed work flow of this research.

Jane Eyre: Bildungsroman

Nicholas Scelzi Mrs. Pinto English 10H Period 2 April 14, 2013 Jane Eyre as a Bildungsroman Jane Eyre, written by Charlotte Bronte, is a Bildungsroman. A Bildungsroman is a novel in which the protagonist engages in a moral and psychological growth. A Bildungsroman generally exhibits the growth and development of a particular individual within a confined social order. The character, to travel on this road to adulthood and development, must have some sort of loss or discontent.The path that the character travels is long, arduous, and gradual and is lodged with hardship and adversity between the needs, desires and views of the protagonist and the norm of society. Ultimately, the spirit and values of the social order becomes evident in the protagonist and the protagonist displays a new position in society. In the opening chapter of Jane Eyre, Jane, the protagonist, is abused emotionally, physically and verbally by her antipathetic family. Her cousin John, who demands that Jane refer to h im as Master, is especially belligerent.While reading and minding her own business, Jane is disturbed and attacked by her malicious cousin for no good reason. This is the first time Jane stands up for herself and yells back at her cousin, only to receive a worse punishment and become imprisoned in the red-room at Gateshead, which is the same room in which her late uncle was waked. She soon hallucinates and sees illusions of her ghastly uncle. Jane later attends an orphan school called Lowood, where she is, yet again, treated cruelly. You can read also Analysis of Literary Devices of Jane EyreHowever, there is a single girl who is content and uncomplaining: Helen Burns, a young girl who has faith that God will. Jane admires this and soon befriends Helen. Helen grows ill and dies, but her last words reassure her strong-rooted faith in God and inspire Jane to have the same faith. Jane remains at Lowood for eight years, six as a student and two as a teacher, furthering her education and accelerating to the top of her class. When Jane leaves Lowood, she gets a job as a governess at Thornfield Hall, where she falls in love with her employer.She discovers, however, that he is already wedded and faces the inner conflict of whether or not to stay with him. She decides it is essential that she must leave and move on. Ten months later, after finding home with her unbeknownst cousins and inheriting a fortune of five-thousand pounds, she returns to her love, Mr. Edward Rochester, and finds out that his insane wife killed hers elf. She accepts his proposal and they marry and live happily thereafter. Jane, who was once an abused orphan, grew to become mature, educated and moral woman. She was a tortured and passionate girl who wanted nothing more than to love and be love. She could not restrain her passion and lashed out at her family for abusing her. However, as the novel progressed, she grew able to control herself, without, though, losing her passion or moral values. She became a giving, selfless, and charitable woman. This shows Jane’s coming of age and development and proves the novel Jane Eyre to be a bildungsroman.

Thirteen Rules of Basketball - James Naismith

Basketball is an original American game invented by Dr. James Naismith in 1891. He designed it with rules of its own. These are the rules published in January 1892 in the school newspaper where he instituted the game. The rules set out a game that is a non-contact sport played indoors. They are familiar enough that those who enjoy basketball over 100 years later will recognize it as the same sport. While there are other, newer rules, these still form the heart of the game. Original 13 Rules of Basketball by James Naismith 1. The ball may be thrown in any direction with one or both hands.Current rule: This is still a current rule, except that now the team isnt allowed to pass it back over the midcourt line once they have taken it over that line. 2. The ball may be batted in any direction with one or both hands, but never with the fist.Current rule: This is still a current rule. 3. A player cannot run with the ball. The player must throw it from the spot on which he catches it, allowance to be made for a man running at good speed.Current rule: Players can dribble the ball with one hand as they run or pass, but they cant run with the ball when catching a pass. 4.  The ball must be held by the hands. The arms or body must not be used for holding it.Current rule: Still applies, it would be a traveling violation. 5.  No shouldering, holding, pushing, striking or tripping in any way of an opponent. The first infringement of this rule by any person shall count as a foul; the second shall disqualify him until the next goal is made or, if there was evident intent to injure the person, for the whole of the game. No substitution shall be allowed.Current rule: These actions are fouls and a player may be disqualified with five or six fouls or get an ejection or suspension with a flagrant foul. 6.  A foul is striking at the ball with the fist, violations of Rules 3 and 4 and such as described in Rule 5.Current rule: Still applies. 7.  If either side makes three consecutive fouls it shall count as a goal for the opponents (consecutive means without the opponents in the meantime making a foul).Current rule: Instead of an automatic goal, sufficient team fouls (five in a quarter for NBA play) now award bonus free throws attempts to the opposing team. 8.  A goal shall be made when the ball is thrown or batted from the grounds into the basket and stays there, providing those defending the goal do no touch or disturb the goal. If the ball rests on the edges, and the opponent moves the basket, it shall count as a goal.Current rule: In the original game, the basket was a basket and not a hoop with a net. This rule evolved into the goaltending and defense pass interference rules. Defenders cant touch the rim of the hoop once the ball has been shot. 9.  When the ball goes out of bounds, it shall be thrown into the field and played by the first person touching it. In case of dispute the umpire shall throw it straight into the field. The thrower-in is allowed five seconds. If he holds it longer, it shall go to the opponent. If any side persists in delaying the game, the umpire shall call a foul on them.Current rule: The ball is now thrown in by a player from the opposite team of the player who last touched it before it went out of bounds. The 5-second rule is still operative. 10. The umpire shall be the judge of the men and shall note the fouls and notify the referee when three consecutive fouls have been made. He shall have the power to disqualify men according to Rule 5.Current rule: In NBA basketball, there are three referees. 11.  The referee shall be the judge of the ball and shall decide when the ball is in play, in bounds, to which side it belongs, and shall keep the time. He shall decide when a goal has been made and keep account of the goals, with any other duties that are usually performed by a referee.Current rule: Timekeepers and scorekeepers now do some of these tasks, while the referee determines ball possession. 12. The time shall be two fifteen-minute halves, with five minutes rest between.Current rule: This varies by the level of play, such as high school and collegiate. In the NBA, there are four quarters, each 12 minutes long, with a 15-minute halftime break. 13. The side making the most goals in that time shall be declared the winner.  Current: The winner is now decided by points. In the NBA, five-minute overtime periods are played in case of a tie at the end of the fourth quarter, with the point total at the end determining the winner. If still tied, they play another overtime period. More: The History of Basketball and Dr. James Naismith

Communication in 12 Angry Men Essay - 793 Words

Reginald Rose’s â€Å"12 Angry Men† is a testament to the power and productivity of conflict. In the same way that conflict can both help and hinder us, the ego/identity and relational based conflicts, and the competitive and avoidance approaches to conflict interfere with the group coming to consensus, yet at the same time galvanize these 12 angry men. Many of the jurors’ personal biases, often the causes of relational or ego/identity based conflict, constantly undermine the voting. Throughout the entire film, perhaps the most heated source of conflict arises from the group’s perception of that era’s underprivileged youth; they are stereotyped as, criminals, menaces to society, and rebels who don’t respect authority. Beginning of film,†¦show more content†¦This case only brings the group off track again as yet another disagreement has to be resolved. While these conflicts themselves might have held the jurors back, the competition approach to these sources of conflict moved the group in the right direction. For example, Juror #9, the old man, approached Juror #10’s insults and stereotyping in competitive fashion, claiming that people are not simply born liars. Later, Juror #10 calls the accused boy a â€Å"common ignorant slob [that] don’t even speak good English,† after which immigrant Juror #11 competitively corrects him. In the end, almost every juror takes a competitive approach to juror #10’s insulting in one of the film’s most powerful scenes as they, one by one, leave the table and turn their backs on him. The jurors unsympathizing intolerance of Juror #10’s bigoted views serve as a prime example of a competitive approach to conflict. The conflict and the approach to the conflict between the jurors and Juror #10 bring the group together as the votes slowly change from guilty to not guilty. Another major source of conflict is the other jurors’ disinterested approach to the trial. Almost every juror approaches Juror #8’s insistence on a not guilty vote with avoidance. They care little about the case and do not grasp its gravity,Show MoreRelatedGroup Communication In 12 Angry Men935 Words   |  4 Pages12 Angry Men: Group Analysis Paper In 1957, the producers H. Fonda, G. Justin and R. Rose collaborated with the director S. Lumet to create the film, 12 Angry Men. In this paper, I will provide an analysis of the small group communication displayed by the main characters in the motion picture. I will discuss group communications, group development, group membership, group diversity, and group leadership. These topics will be dissected in order to properly examine the characters’ behavior. 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